Tony-award winner Menzel, who provides the English voice for Queen Elsa in the film, was joined by her co-stars from across the world for the special performance at the awards show.
Alongside Menzal, those performing included West End star Willemijn Verkaik, who provided the German voice of Elsa, together with Maria Lucia Heiberg Rosenberg (Danish), Takako Matsu (Japanese), Carmen Sarahí (Spanish), Lisa Stokke (Norwegian), Kasia Łaska (Polish), Anna Buturlina (Russian), Gisela (Castilian) and Gam Wichayanee (Thai).
Also singing with the group was Norwegian singer AURORA, who provides a haunting voice in both the song and throughout the second film.
You can watch the Into the Unknown Oscars performance featuring Idina Menzel below...
Into The Unknown was nominated for Best Original Song at the awards but lost out to Rocketman's (I'm Gonna) Love Me Again written by Elton John and Bernie Taupin.
It's one of the brand new songs written by husband and wife team Robert Lopez and Kristen Anderson-Lopez that feature in the sequel to the 2013 movie, which was release in cinemas last year.
The film was a huge hit, surpassing the original to make almost $1.5 billion worldwide making it the highest grossing animated movie of all time after the original Frozen.
The full Frozen 2 soundtrack is available now on CD, download and streaming.
Meanwhile, a stage adaption of the original film is currently running on Broadway and will open in London's West End later this year.