The Mousetrap will reopen in the West End with two separate casts.
It was previously reported that socially distanced West End performances of the play would resume in October.
After performances were halted in March amid lockdown restrictions, the show has announced plans to start again from October 23.
Shows will feature two alternating eight-member casts to ensure the show can go on amid the pandemic.
One cast features Kate Tydman (Mollie Ralston), Haydn Oakley (Giles Ralston), Alexander Wolfe (Christopher Wren), Brenda Longman (Mrs Boyle), Peter Landi (Major Metcalf), Lizzie Muncey (Miss Casewell), Philip Voyzey (Mr Paravicini) and Paul Hilliar (Detective Sergeant Trotter).
The other includes Eleanor McLoughlin (Mollie Ralston), Neil Ditt (Giles Ralston), Joshua Griffin (Christopher Wren), Nicola Blackman (Mrs Boyle), Damien Matthews (Major Metcalf), Sarah Moss (Miss Casewell), Tony Timberlake (Mr Paravicini) and Sam Ebenezer (Detective Sergeant Trotter).
The piece will be presented with social distancing measures on stage, backstage and in the audience with a capacity of 200.
The Mousetrap is famous around the world for being the longest running show of any kind in the history of British theatre.
The Mousetrap is the iconic murder mystery play. The scene is set when a group of people gathered in a country house cut off by the snow discover, to their horror, that there is a murderer in their midst. Who can it be? One by one the suspicious characters reveal their sordid pasts until at the last, nerve-shredding moment the identity and the motive are finally revealed.
In her own inimitable style, Dame Agatha Christie has created an atmosphere of shuddering suspense and a brilliantly intricate plot where murder lurks around every corner.