The Play That Goes Wrong will resume performances in London's West End from November 19.
The hit comedy play will reopen at the Duchess Theatre where it first started performances in September 2014.
The show will follow the latest government guidelines including a reduced capacity audience and social distancing.
The cast will be made up of David Kirkbride, Ross Green, Ciara Morris, Milo Clarke, Michael Keane, Blayar Benn, Elan James and Ellie Morris. The understudies are Tom Bulpett, Oliver Clayton, Antonia Salib and Jack Michael Stacey.
Producers Kenny Wax and Stage Presence Ltd said: "The Play That Goes Wrong has been a tonic for people's spirits since first appearing seven years ago. We know how many thousands of performances have been on stage, how many people have bought tickets and how many hundreds of people have been employed.
"Laughter has the power to bring people together across all divides, reduces tensions and produces joy. And we know how lacking all of those have been these challenging past six months.
"So we count ourselves fortunate that our show is of a size where it is possible to re-open now, and while playing to the smaller-sized audience that social distancing requires, costs can just about be covered. Tickets from £15, Laughs priceless."
Alongside the West End restart, The Play That Goes Wrong's UK tour will be playing a limited Christmas season at Theatre Royal Bath, from December 17 to January 10.
Producer Kenny Wax continued: “We are one of a handful of small / middle scale productions who have low enough running costs to open with a socially distanced audience and even though we are unlikely to be able to make a profit we will be employing about 60 staff across the two productions in London and Bath."
He added that the productions were "still taking a significant risk without a government backed insurance scheme to cover further business cancellation" due to the pandemic.
"Our continual asks of government are (1) lift social distancing (2) provide a government backed insurance scheme," Wax concluded.